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《Periodical of Ocean University of China》 2015-01
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A Methodological Study on the Induction of Triploidy Oyster with Different Salinities

YU Rui-Hai;WANG Zhao-Ping;KONG Jing;SHEN Jian-Ping;ZUO Zi-Rong;College of Fisheries,Ocean University of China;Shangdong Haiyibao Aquatic Products Co.,LTD;  
Triploidy Crassostrea gigas was induced by inhibiting polar bodyⅡ(PB2)releasing with low salinities(4,6,8,10,12,14 and 16)and high-salinities(40,45,50,55,60,65 and 70)when 40%~50% of PB1 were observed.The inducing time was 10 and 15min,rewspectively.The results showed that triploid was inducible with with both low and high salinities.When the salinity converged to the natural,hatchery rate increased.The difference of cleavage rate among low salinities and reducing times was significant(P0.05)while the difference of hatchery rate was similar(P0.05).Flow cytometry analysis showed that the highest triploidization rate was 84.4%(10min)and 91.0%(15min)at lowsalinities(S=8),and the difference of triploidization rate was significant(P0.05).The differences of cleavage and hatchery rates of high salinities were significant(P0.05).The highest triploidization rate were 80.3%(S=55,10 min)and 97.1%(S=60,15 min),and the difference of triploidization rate was significant(P0.05).The zygotes of C.gigasinduced for 15 min at 8and 60 salinities when40%~50% of PB1 appeared could yield the highest triploidy.
【Fund】: 国家高技术研究发展计划项目(2012AA10A412);; 广东省部产学研结合专项资金项目(2012B091000102)资助
【CateGory Index】: S968.3
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