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《Periodical of Ocean University of China》 2017-08
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Effect of Thermal Stimulus on Immune Function and Heat Shock Protein Expression of Scallop Chlamys farreri

LIU Tian-Yu;WANG Qing;CHEN Mu-Yan;The Key Laboratory of Mariculture(Ocean University of China),Ministry Education;The Key Laboratory of Coastal Zone Environment Processes and Ecological Remediation,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences;Muping Coastal Environment Research Station,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences;  
The objective of this study was to evaluate the effect of thermal stimulus on the scallop,Chlamysfarreri,which was generally recognized as a temperature sensitive bivalve species.Immune response,DNA damage,apoptosis and transcription of heat shock protein(HSP70and HSP90)genes in hemocytes were evaluated over a short period of thermal stimulus.The hemocyte of scallops was obtained on 0,1,2,4,8hafter being treated at 28℃.By using flow cytometry,reactive oxygen species(ROS)production and phagocytosis activity of hemocytes were estimated.The results demonstrated that both ROS and phagocytosis activity reached the highest level in 4hand reduced markedly by 8h.The stress also significantly decreased antibacterial and bacteriolytic activities.By contrast,the DNA damage(mainly DNA breakages)level in hemocytes as was detected by the alkaline comet assay increased following high-temperature exposure,and hemocyte apoptosis was observed under fluorescence microscope.Significant increases in the transcription of HSP70 and HSP90genes were also detected in hemocytes,while the transcription of HSP70 grew faster than HSP90 did and reached to a higher level.This study demonstrated that immune response,DNA damage level and apoptosis of hemocytes in the scallop were strongly affected within several hours,and the transcripts of HSPs genes were strengthened so as to protect the cells and tissues from injury when C.farreri subjected to short-term high temperature stressing.
【Fund】: 山东省自然科学基金项目(ZR2012CQ016);; 中国科学院海洋生态与环境科学重点实验室开放基金项目(KLMEES201301);中国科学院青年创新促进会项目(2016196)资助~~
【CateGory Index】: S917.4
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【Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Caili;WANG Xiuhua;WANG Dongsheng;SHEN Jian;TENG Yu;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;Shandong Weifang Longwei Industrial Limited Company;Fishery Machinery and Instrument Research Institute, Chinese Academy of Fishery Sciences;;Sustainable Development of Scallop Industrial Integration[J];天津农业科学;2016-02
2 QIAN Jiahui;LI Zhimin;SHEN Yuchun;LIU Zhigang;ZHANG Wucai;Fisheries College,Guangdong Ocean University;;Synergistic effect of temperature and salinity on antioxidant enzymes activities of Chlamys nobilis[J];南方水产科学;2015-06
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4 LIAN Shan-Shan;LI Xue;XING Qiang;ZHAO Bo-Song;ZHANG Ling-Ling;BAO Zhen-Min;The Key Laboratory of Marine Genetics and Breeding,Ministry of Education,Ocean University of China;;Effects of Reproduction and Heat Stress on the Antioxidant Ability of Zhikong scallop(Chlamys farreri)[J];中国海洋大学学报(自然科学版);2015-10
5 XU Yi-Xuan;ZHAN Wen-Bin;XING Jing;The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China;;Effect of Water Temperature on Phagocytic Activitiy of the Haemocytes of Scallop(Chlamys farreri)After Stimulated by Lipopolysaccharide[J];中国海洋大学学报(自然科学版);2015-06
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【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 LIU Tian-Yu;WANG Qing;CHEN Mu-Yan;The Key Laboratory of Mariculture(Ocean University of China),Ministry Education;The Key Laboratory of Coastal Zone Environment Processes and Ecological Remediation,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences;Muping Coastal Environment Research Station,Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences;;Effect of Thermal Stimulus on Immune Function and Heat Shock Protein Expression of Scallop Chlamys farreri[J];中国海洋大学学报(自然科学版);2017-08
2 Liu Aidong;Wang Qing;Tian Lin;Liu Xiaohong;Zeng Junwei;Department of Physiology,Zunyi Medical University;Function Laboratory of Zunyi Medical University;;Influence of exogenous hydrogen sulfide on cell apoptosis induced by high glucose in human proximal renal tubular epithelial cells[J];遵义医学院学报;2017-03
3 LIU Qiang;LI Jian-he;Department of Pharmacy, Second Xiangya Hospital, Central South University;;Chinese herbal medicines as apoptosis inducers for cancer treatment[J];中南药学;2017-06
4 Wei Xiang;Chen Shaolong;Jia Ning;Laboratory of Molecular Biology,Anhui Medical University,Key Laboratory of Gene Resource Utilization for Genetic Diseases of Ministry of Education and Anhui Province;;Effect of all-retinoic acid on the apoptosis of human gastric cancer SGC-7901 cells[J];安徽医科大学学报;2017-08
5 YANG Dong-min;ZHANG Yan-li;DING Jian-feng;YANG Feng;HUO Zhong-ming;NIE Hong-tao;YAN Xi-wu;College of Fisheries and Life Science,Engineering Research Center of Shellfish Culture and Breeding in Liaoning Province,Dalian Ocean University;;Synergistic effects of high temperature and low salinity on immunity of Manila clam Ruditapes philippinarum[J];大连海洋大学学报;2017-03
6 ZHAO Meng;ZHU Rui;CHEN Yulong;Basic Medical College,Henan University of Traditional Chinese Medicine;;Interpretation of the Research Progress on the Development and Treatment of Cancer with the Theory of Yin and Yang[J];光明中医;2017-09
7 ZHENG Yao;LIU Jun-rong;ZHOU Yan-lin;LIU Hui-hui;SONG Yang;TIAN Yuan-yong;ZHAO Xue-wei;College of Food Science and Engineering,Dalian Ocean University;Zhangzidao Group Company Limited;;Immunologic factors in adductors and quality evaluation in live and air exposure and wet stored Yesso scollap Patinopecten yessoensis[J];大连海洋大学学报;2017-02
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9 LOU Ye-liang;CHEN Meng-jing;WANG Ke;GONG Xue-yuan;GONG Heng-pei;ZHONG Xiao-ming;CHENG Ru-bin;HUANG Zhen;Zhejiang Chinese Medical University,College of Pharmacy;;Effects of harpagide on cerebral ischemia and mitochondria mediated Caspase dependent apoptotic signaling pathway in mice[J];中国药理学通报;2017-04
10 ZHANG Xi-kai;YU Guang-qing;NI Lei;The First Hospital Affiliated to Jiamusi University;;The Effect of Wee1 Inhibitor Combined with Adriamycin on HL-60 in Acute Leukemia Cell Line[J];中国医学创新;2016-36
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 LI Juan;YU Fei;GUAN Chang-tao;SHEN Jian;TENG Yu;WANG Cai-li;Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences;Fishery Machinery and Instrument Research Institute, Chinese Academy of Fishery Sciences;;Sustainable Development of Shellfish Industrial Integration in Shandong[J];天津农业科学;2015-07
2 SUN Yu;MU Yong-tong;Ocean University of China;Qingdao Agricultural University;;Chinese status in the world scallop industry:production,output,value perspective[J];中国渔业经济;2014-04
3 HAO Zhen-lin;TANG Xue-jiao;DING Jun;BEN Yue;CHANG Ya-qing;Key Laboratory of Mariculture & Stock Enhancement in North China’s Sea,Ministry of Agriculture of China,Dalian Ocean University;;Survival rate,oxygen consumption rate and immune enzymetic activity of Mizuhopecten yessoensis at high temperature[J];生态学杂志;2014-06
4 LV Hao-ze;LIU Jian;CHEN Jin-hui;LI Duo;SHEN He-ding;College of Fisheries and Life Science,Shanghai Ocean University;Management Office of Shanghai Yangtze Estuary Chinese Sturgeon Natural Reserve;;Effects of salinity on activities of SOD and CAT in hepatopancreas of Sinonovacula constricta[J];海洋渔业;2013-04
5 ZHANG Xiao-li;HU Yu-zhen;LI Ming-yun;MIAO Liang;CHEN Jiong;SHI Yu-hong;LU Xin-jiang;Key Laboratory of Applied Marine Biotechnology, Ministry of Education, School of Marine Sciencies, Ningbo University;;Changes in antioxidant level and serum enzyme activity of farmed large yellow croaker during natural water cooling in winter[J];海洋科学;2013-11
6 WANG Chong;Wafangdian Ocean and Fisheries Bureau;;Comparison and Path Analysis of Important Economic Traits between Genders of Scallop Chlamys farreri[J];水产科学;2013-08
7 YU De-liang;DING Jun;HAO Zhen-lin;LI Sheng-de;CHANG Ya-qing;Key Laboratory of Mariculture & Stock Enhancement in North China's Sea,Ministry of Agriculture,Dalian Ocean University;College of Science,Dalian Ocean University;;Correlation and path analysis of quantitative traits in different cultured populations of yesso scallop Patinopecten yessoensis[J];大连海洋大学学报;2013-04
8 TENG Yu1,LI Juan1,WANG Zhi-yong1,*SHEN Jian2,WANG Cai-li1(1.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,Shandong 266071,China; 2.Fishery Machinery Instrument Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200092,China);The Dietetic Therapy Nutrition and Utilization of Ark Shells[J];农产品加工(学刊);2013-13
9 WANG Cai-li1,LI Juan1,WANG Bin-ting2,WANG Zhi-yong1,SHEN Jian3,TENG Yu1,*(1.Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao 266071,China;2.Shandong Weifang Longwei Group Ltd.,Werifang 262737,China;3.Fishery Machinery and Instrument Research Institute,Chinese Academy of Fishery Sciences,Shanghai 200092,China);Study on Processing Technology of Maoctra veneriformis Instant Food[J];保鲜与加工;2013-04
10 SHI Shaokun1,2,WANG Ruixuan1,WANG Jiangyong1,JIANG Jingzhe1,LIU Guangfeng1,YANG Rui1,2(1.Key Lab.of Fishery Ecology and Environment,Guangzhou Province;Key Lab.of South China Sea Fishery Resources Exploitation & Utilization,Ministry of Agriculture;South China Sea Fisheries Research Institute,China Academy of Fishery Sciences, Guangzhou 510300,China;2.College of Fisheries and Life,Shanghai Ocean University,Shanghai 201306,China);Effects of salinity stress on immune factors of Crassostrea hongkongensis[J];南方水产科学;2013-03
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