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《Practical Journal of Medicine & Pharmacy》 2010-01
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Efficacy of propofol on long-term depression in the CA1 region of rat hippocampus

LI Xiao-qiang,SUN Xue-hua.Department of Anesthesiology,No.107 Central Hospital of PLA,Yantai,SHandong 264000,China  
Objective To evaluate the impact of propofol on excitatory synaptic responses and synaptic plasticity in rat hippocampus.Methods Male Wistar rats(3 week old)were decapitated rapidly and the hippocampal brain slices were cut coronally in 400 μm.The microscope was used to identify the CA1 region of the hippocampus.With electrical stimulation on Schaffer collaterals,the excitatory post-synaptic current(EPSC)were recorded using blind patch-clamp technique;after 10min recording of EPSC baseline,propofol was perfused in bath solution with different concentrations.Then EPSC was continously recorded for another 30min.The impact of propofol on synaptic plastisity after 10min recording of EPSC baseline was observed after low frequency stimulation(LFS,0.3Hz,900pulse)was used to induce LTD.Then the slice was incubated with propofol for 0.5h and LTD was induced as above.Results Propofol dose-dependently inhibited EPSC,this effect can be completely blocked by picrotoxin.Propofol also facilitated LTD induction,which was depended on NMDA receptors.Conclusion Propofol can affect excitatory synaptic transmission and synaptic plasticity in the CA1 area of the rat hippocampus,so it can affect learning and memory in rats.
【CateGory Index】: R96
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