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《Journal of Qufu Normal University(Natural Science)》 2013-04
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Genetic Diversity of the Wild Elymus sibiricus L.Germplasm Resources Detected by SSR Markers

WU Hao;QU Zhi-cai;YAN Wei-hong;ZHANG Xiao-qing;MA Yu-bao;WANG Kai;QI Juan;College of Life Sciences,Qufu Normal University;Institute Grassland Research of CAAS;Gansu Agricultural University;  
Genetic diversity of 35 wild Elymus sibiricus germplasm resources from seven sample points of five union city in Inner Mongolia region was evaluated by using SSR markers.Twenty pairs of the SSR polymorphic primers were used in the SSR analysis.A total of 326 bands were detected,the average was 12.55 polymorphic bands per primer pair;the percentage of polymorphic was 76.99% and the genetic distance ranged from 0.0000 to 0.3079,indicating that the relationship amnong all individuals was close;The Nei's genetic diversity index(h)of 35E.sibiricus germplasm was 0.2223,the Shannon index(I)was0.3288.These results indicated that the genetic diversity among the tested materials are relatively abundant.The E.sibiricus could be classified into two groups at genetic similarity 0.71.The E.sibiricus materials collected from the eastern,central and western areas of Inner Mongolia showed cross-clustering characteristics,and they exhibited no relationship with their geographical distribution.This study has a theoretical basis to explore the sibiricus genetic variation relationship with the environment and E.sibiricus wild germplasm resources protection.
【Fund】: 中央级公益性科研院所基本科研业务费专项(1610332012209);; 内蒙古自治区自然科学基金项目“优异老芒麦耐旱性研究”(2011BS0411)
【CateGory Index】: S543.9
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