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《Journal of Qufu Normal University(Natural Science)》 2018-04
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Clone and Bioinformatic Analysis of Htr1a in Cricetulus Barabensis

GAO Gui-bin;XU Jin-hui;XUE Hui-liang;WU Ming;XU Lai-xiang;College of Life Sciences,Qufu Normal University;  
Serotonin 1 A(5-hydroxytryptamine receptor 1 A,Htr1 a)is the key to mediate the 5-HT system function,which distributed mainly in the frontal cortex,hippocampus,lateral septum,dorsal raphe nucleus,spinal anterior horn.Based on the striped hamsters(Cricetulus barabensis)for materials,the cDNA sequence of Htr1 awas cloned by RT-PCR.The nucleic acid sequences and protein sequences of Htr1 awere analyzed by bioinformatics.The results show that the sequence is 1486 bp in length,containing part of the 5′noncoding region,part of the 3′noncoding region and a complete open reading frame of1269 bp which encodes a polypeptide of 422 amino acids residues(GeneBank ID:MH169583).The Htr1 a protein has a molecular weight of 46332.47 Da and an isoelectric point of 9.18.The protein has 7 transmembrane helices,a G protein-coupled receptor family tag,no signal peptide,doees not belong to a secreted protein,and hydrophobicity analysis also shows that it conforms to the membrane protein characteristics.Homology comparison and phylogenetic tree analysis show that the striped hamster(Cricetulus barabensis)is closest to the Chinese hamster(Cricetulus griseus)and related to rodents more closely,but has a distant relationship with other mammals and bird species,which is consistent with traditional species taxonomy.The above results confirm that this experiment cloned part of the cDNA sequence of the striped hamster Htr1 asuccessfully,which lay a foundation for further exploration of the function of 5-HT system in the striped hamster.
【Fund】: 国家自然科学基金(31670385 31570377 31770455)
【CateGory Index】: Q78;Q811.4
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