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《Qinghai Medical Journal》 2003-01
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AN EXPERIMENTAL STUDY OF ADHESION OF PMVEC AND PMN AND MOLECULAR MECHANISM OF NF-KAPPA B ACTIVATION INDUCED BY LPS

Liu Xiaoli,et al The General Hospital of Tibet Military Command  
To study the adhesion of pulmonary microvascular endothelial cells(PMVEC) to polymorphonuclear neutrophils(PMN)induced by LPS and its mechanism.Method:To measure the adhesion rate of PMEVC-PMN and ICAM-lexpression stimulated by 100ng/ml LPS at Oh,2h,4h,6h,8h or 10ng/ml,50ng/ml,100ng/ml LPS at 6h and the activation of NF-κB by EMSA.Above results were compared with which was modulated by anti-ICAM-1 or PDTC.Results:The expression of ICAM-1 and the adhesion rate of PMVEC-PMN were highly depended on the stimulation about the dose and time of LPS.And NF-κB was rapidly activated by LPS stimulation and peaked at 1h,then lowed.The anti-ICAM-1 or PDTC could significantly decreased the adhesion of PMVEC-PMN( P0.01 ).Conclusion:The activated NF-κB by LPS starts up ICAM-1 expression and increases the adhesion of PMVEC-PMN.
【CateGory Index】: R363
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