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《Qinghai Medical Journal》 2003-01
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Liu Xiaoli,et al The General Hospital of Tibet Military Command  
To study the adhesion of pulmonary microvascular endothelial cells(PMVEC) to polymorphonuclear neutrophils(PMN)induced by LPS and its mechanism.Method:To measure the adhesion rate of PMEVC-PMN and ICAM-lexpression stimulated by 100ng/ml LPS at Oh,2h,4h,6h,8h or 10ng/ml,50ng/ml,100ng/ml LPS at 6h and the activation of NF-κB by EMSA.Above results were compared with which was modulated by anti-ICAM-1 or PDTC.Results:The expression of ICAM-1 and the adhesion rate of PMVEC-PMN were highly depended on the stimulation about the dose and time of LPS.And NF-κB was rapidly activated by LPS stimulation and peaked at 1h,then lowed.The anti-ICAM-1 or PDTC could significantly decreased the adhesion of PMVEC-PMN( P0.01 ).Conclusion:The activated NF-κB by LPS starts up ICAM-1 expression and increases the adhesion of PMVEC-PMN.
【CateGory Index】: R363
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