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Relationship between shear stress and the proliferation and differentiation of artery smooth muscle cells

Ying Wang, Yubo Fan, Anqiang Sun (Biology Engineering Department of Beihang University, Beijing,100083,China)  
Objective: To investigate effects in the proliferation and differentiation of human umbilical artery smooth muscle cells(HUASMC) on the shear stress. Methods: Using the mended filled and parallel plate flow apparatus and pump providing fluid shear stress, human umbilical artery smooth muscle cells(HUASMC) culture models were established in vitro with the same environment factors as steady culture. Load the different team of VSMC with 3,4,5,8,10 dyn/ cm2 steady fluid sheer stress for 24h ,the cells in steady culture as the control .The cells are observed and counted in the discrepancy inversion microscope, and the samples are disposed in α-actin immunohistochemical. Results: Compare to the control. the capability of proliferation in all the teams under sheer stress is decent, the most obviously is the sample with 5 dyn/ cm2. The result ofα-actin immunohistochemical show that the cells cultured with the sheer stress have more deep Conclusion: Pilot study suggested the sheer stress restrain the proliferation of human umbilical artery smooth muscle cells, and might promote the differentiation.
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