The Decrease of Doaminergic Neurons and the Activation of Glias IN Rat Parkinson's Disease Model Induced with 6-Hydroxydopamine
Zhang Si-yuan Qi Jian-guo Zhang Wei He Yi (Department of Histology, Embryology and Neurobiology, West China School of Preclinicaand Forensic Medicine,Sichuan University, Chengdu 610041) (Department of Neurology, Baylor College of Medicine, Houston TX 77030, USA)
Objective To create the Parkinson's disease rat model by using 6-hydroxydopamine(6-OHDA). Methods 6-OHDA was injected into unilateral intramedial forebrain bundle(MFB). The numbers and morphology of dopaminergic neurons, microglias and astrocytes were examined by immunohistochemistry in the substatia nigra(SN) of all animals at 3th, 7th and 14th day following 6-OHDA injection. The pictures were got with the special microscope camera and the grey means were examined with the analysis software of computer. Results The number of the TH immunoreactive neurons decreased in the ipsilateral SN comparing with the controlateral.and the grey mean of lesioned SN increased. At the same time, the microglias and astrocytes increased obviously in number, and the grey mean of the lesioned SN decreased. All the differences are significant statistically in groups (P0. 01). The morphology of the active microglias and astroglias variered prominently. Conclusion The PD animal model could be made successfully by injection of 6-OHDA into the MFB.