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The Klebsiella Pneumoniae Clearance Enhanced by Adenovirus Vector Encoding Mutant Rip2 Gene in Human Urinary Epithelial Cell

LIU Xing-hai,LIU Wei,SUI Peng-nuo,HUANG Ning,WANG Bo-yao,WU Qi(Institution of Infection and Immunity,West China Medical Center,Sichuan University,Chengdu 610041)  
Objective To investigate the function of Rip2 gene on urinary infections induced by Klebsiella pneumoniae.Methods Hunman bladder carcinoma cell line T-24 was transfected with adenovirus expression vector containing both mutant Rip2 gene and green fluorescent protein(GFP) gene,and the cells transfected with adenovirus expression vector merely containing GFP gene used as control.Their transfected efficiency was indicated by GFP expression.Klebsiella pneumoniae clinical isolated 5 is incubated with these transfected cells at 37℃.At 4,24 and 48 hour after initial incubation,viable intracellular bactria were quantified by plating appropriate dilution on LB agar plates.Results Compared with the T-24 transfected with GFP gene,the ability of clearing Kp5 in T-24 transfected with mutant Rip2 gene decreased significantly(P0.01).Conclusion Mutant Rip2 gene could resist the effect of clearing Klebsiella pneumoniae in bladder epithelial cell,implying that Rip2 gene may serve as an innate immune receptor recognizing urinary pathogens.
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