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《Sichuan Journal of Anatomy》 2010-01
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Expression of Toll-Like Receptor 4 and NF-κ Bp50 in Experimental Allergic Rhinitis in Rats

Zhou Lin,Zheng Ming(Centrer of Neuroscience,Fujian Medical Univesity,Fujian Fuzhou 350004)  
Objective To observe the expression of toll-like receptor 4 and NF-κ Bp50 in experimental allergic rhinitis(AR) and study the effect of lipopolysaccharide(LPS) and dexamethasone on their expression.Methods 48 SD rats were divided randomly into four groups:AR group(group A);Allergic rhinitis + LPS administration group(group B);Normal control group;Allergic rhinitis + dexamethasone group(group D).The nasal mucosae were studied by Wright's staining to observe the infiltration of inflammatory cells.The numbers of eosinophile granulocytes in the epitheliumwere counted under the high power light microscope.The expression of TLR4 and NF-κB p50 proteins was detected by western-blotting and immunohistochemical staining respectively in rat nasal mucosa of all the groups.Results The animal models of allergic rhinitis were made sucessfully in group A,B,D.The numbers of eosinophils: group B was significantly higher than in group A、C、D(p0.01),and significantly higher in group A than in groupC、D(p0.01),group C and D showed no significant difference(P0.05).ICH staining:NF-κBp50 expressed in all the groups,the expression of NF-κBp50 in group B was significantly higher than in group A、C、D(p0.01),and significantly higher in group A than in groupC、D(p0.01),group C was significantly higher than group D.western-blotting: TLR4 expressed in all the groups,the expression of TLR4 in group B was significantly higher than in group A、C、D(p0.01),and significantly higher in group A than in groupC、D(p0.01),group C and D showed no significant difference(p0.05).Conclusion The expression of TLR4 and NF-κBp50 increased in rat nasal mucosa with experimental allergic rhinitis.The expression of TLR4 and NF-κBp50 in allergic rhinitis rats can be futher increased by LPS and decreased by the intervention of dexamethasone.It suggests that TLR4 and NF-κBp50 have an effect on allergic inflammation.Dexamethasone may decrease the expression of TLR4 and NF-κBp50,which may correlate with the development of allergic rhinitis.
【CateGory Index】: R765
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