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Determination of Phillyrin of Dujiaoliangao by HPLC

ZHU Jun-de1,YU Zi-jiang1,GONG Ming2(1.Department of Anatomy,Guiyang Medical College,Guiyang 550004;2.Department of Drug Research Guizhou Yibai Pharmacy Co Ltd,Guiyang 550002,China)  
Objective To establish a method to determine the content of phillyrin of Dujiaoliangao by HPLC.Methods The column used in the analysis was Hypersil ODS2(5.0×200 mm)with the temperature of 30℃ and detected at 227nm,with the mobile phase of acetonitrile and water(24: 76).The flow rate of the mobile phase was 1.0ml/min.Results The linear range of phillyrin was 0.1424μg~0.712μg(r= 0.99998).The average recovery rate was 99.22% with a RSD of 2.16%.Conclusion The method is accurate and simple with a good reproducibility,and can be used to determine the content of phillyrin of Dujiaoliangao.
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