Determining the DNA Damage of Crucian Hepatic Cell Induced by Potassium Dichromate
Rao Chaolong Liu Tianqiang Huang Guanjun Liu Yan-peng Xiao Dan1 (Chengdu University of Traditional Chinese Medicine,Chengdu 610075,China)2 (Tongwei Co.,Ltd,Chengdu 610041,China)
Objective To develop the comet assay of Crucian hepatic cells and to determine the cells' DNA damage induced by Potassium Dichromate. Methods The Crucian hepatic cells' solution was prepared and treated with Potassium Dichromate for 1.5 h,at concentration of 0.5,0.25 and 0.1mM respectively,then determined with comet assay. Results There had significant difference of tail length between the experimental group at concentration of 0.5mM or 0.25mM and group of negative control (P0.05). Dose-response relationship had also been observed. Conclusion Comet assay of Crucian hepatic cells had been developed successfully. Potassium Dichromate can cause DNA damage of Crucian hepatic cells.