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The Synaptonphysin Expression in Hippocampal of the Hypoxic-Ischemic Brain Damaged Neonatal Rat and the Regulatory Effect of Angelica Sinensis

Wu Lin1 Tian Long1 Gou Lanying1 Feng Jiayue1 Yu Hong21(Grade 2006,Luzhou Medical College,Luzhou 646000,China)2(Department of Histology and Embryology,Luzhou Medical College,Luzhou 646000,China)  
Objective To explore the expression of synaptophysin(SY) in hippocampal dentate gyrus neurons of the neonatal rats induced by hypoxic-ischemic brain damage(HIBD),and to study the regulatory effect of Angelica sinensis injection on SY expression.Methods The seven-day-old healthy Sprague Dawley rats(n=33) were divided into three groups,namely control group(CG,n=11),hypoxia group(HG,n=11),and Angelica group(AG,n=11).The left carotid artery of HG and AG rats were ligated and placed into the three-gas incubator for two hours to make the HIBD model of neonatal rats,CG only was received a Sham-operation without carotid artery ligation.Following the eight day operation,HG and CG were received intraperitoneal normal saline injection(8 ml/kg) for 7 days.And AG was made the same as HG during procedures,but injected Angelica sinensis instead of normal saline injection.The neonatal rats of each group were fed for 22 days.The 22-day-old juvenile rats,brain tissue of juvenile rats were taken,paraffin-embedded and sectioned through hippocampal.The expression of SY was detected with immunohistochemistry technology and the IOD of SY positive cells in hippocampal dentate gyrus neurons was analyzed.Results The IOD value of the SY positive cells in HG was lower than that of CG,while the IOD value of SY positive cells was higher than that of HG.Conclusion Angelica sinensis injection may play a protective role in the expression of synaptophysin during inhibition on hippocampal dentate gyrus neurons induced by HIBD.
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