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Study on the Expression of HIF-1α in Entochondrostosis

Jin Xin Mao Caiying Wang Song(College of Health Science,Wuhan Sports University,Wuhan 430079,China)  
Objective To investigate the expression of HIF-1α in entochondrostosis,and to study it's role in the process of vascular intrusion.Methods The morphology change in rat's femoral bone development were observed using HE staining method.The protein expression of HIF-lα and VEGF in femoral bone was detected by immunohistochemistry,and the mRNA expression of HIF-lα and VEGF was detected by reverse transcription-polymerase chain reaction(RT-PCR) method.Results Many cartilage cells were seen in femoral bone at the 1st day after the birth;many vessels began to invade at the 7th day;at the 13th day,the cartilage matrix began to calcification,vessels and cartilage cells were reduced.The protein expression of HIF-lα and VEGF was increased at the 7th day,while decreased at the 13th day.The mRNA expression of HIF-lα and VEGF went up to the maximum at 7th day the time,and then decreased.Conclusion During femoral bone development,hypoxia may induce HIF-1 expression,then activates it's down stream gene VEGF expression,which would induce the intrusion of exogenous vascular,promote endochondral osteogenesis.
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