The Influence of Salvia Militiorrhiza on Acute Seizure Model Rat
Liu Baojun Bei Bei Bai Yunchi Kan Zhisheng Liu Feng(Nerosurgery Department,Hospital of Kailuan Co.,Ltd,Tangshan 063000,China)
Objective In order to investigate the effect of Salvia militiorrhiza(SMR) on the acute seizure model,the behavior,electroencephalogram of the model and the expression of GFAP were observed within rat brain during the episode of epilepsy and after treatment.Methods 24 rats were divided into three groups randomly: PTZ(pentylenetetrazol) induced seizure group(10 rats),SMR treatment group(10 rats) and normal control group(4 rats).PTZ induced seizure group's rats were received a dose of 75 mg/kg/body weight PTZ intraperitoneally;Parenteral solution of SMR(10ml/kg) were injetcted into the SMR treatment group's rats abdominal cavity just 30 minutes prior to the injection of PTZ.Normal control group's rats were received same volume saline instead of PTZ.Behavior observation and EEG were observed through the behavior of the rats after injection of PTZ.Immunohistochemistry was performed on the GFAP-positive cells within hippocampus.Results(1) Behavior changes: The stages of seizure and the duration of convulsion of the SMR group were lower than that of PTZ group(P0.05).(2) EEG records contained obvious epileptic electric discharge in PTZ group including abnormal spikes,polyspike discharge,and spike-wave complexes.The EEG records of SMR group contained epileptic electric discharge in a low degree,it showed intervallic polyspike discharge.(3) Immunohistochemistry results was shown that: ①24 h group: Compare with normal control group,the expression of GFAP within hippocampus of PTZ group was obviously increased,especially seen in dentate gyrus and CA3 region,wherein GFAP-positive cells had more branches,along with dense staining compare with PTZ group;the expression of GFAP in SMR treatment group was increased,wherein GFAP-positive cells' number and length were both increased.The staining of them was more condense than PTZ group.② 72h group: The expression of GFAP has no significant difference among PTZ group,SMR group and normal control group.Conclusion The inhibition of SMR on acute epileptic spasm is obvious;The increased expression of GFAP within hippocampus 24 hours after seizure perhaps has protection for animals,which suggests the GFAP perhaps takes part in SMR therapeutical effect.