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《Sichuan Journal of Anatomy》 2012-01
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Effect of Trichostatin A on the human colon carcinoma HCT116 cells

Wang Li1 Liu Xiaoyan1 Li Juan1 Yang Zhihui2 1(Laboratory of Medical Molecular Biology,Luzhou 646000,China) 2(Department of Pathology,Luzhou Medical College,Luzhou 646000,China)  
Objective To investigate the effect of trichostatin A(TSA) on colon carcinoma,and to explore the mechanism of TSA on the proliferation and apoptosis of human colon carcinoma cell HCT116.Methods MTT,Hoechst,Western blot and optical microscope were conducted to observe the alteration of cell proliferation,apoptosis of HCT116 after treated with TSA.Results The proliferation of HCT116 was inhibited by TSA in a time-dependent manner while not in dose dependent manner.TSA induced the apoptosis of HCT116 cells by increasing the expressions of fragile histidine triad(FHIT) and Bax,decreasing the expressions of Osteopontin(OPN).Conclusion The proliferation of HCT116 cells could be inhibited by TSA,the mechanisms of cell apoptosis maybe relate to upregulating FHIT,Bax expression and downregulating OPN expression.
【Fund】: 四川省科技厅科研项目(No.07JY029-134);; 四川省教育厅科研项目(No.07ZB110)
【CateGory Index】: R735.35
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