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《Sichuan Journal of Anatomy》 2012-01
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A simple method of Schwann cell cultures

Zhang Hua1,2 Shi Shuyan3 Liu Jia4 Wang Tinghua4 Yang Hongying1 1(The Second Affiliated Hospital of Kunming Medical University,Kunming 650101,China) 2(Dongfeng Affiliated Hospital of Hubei Medical College,Shiyan 442000,China) 3(College of Basic and Forensic Medicine,Sichuan University,Chengdu 610041,China) 4(Institute of Neuroscience,Kunming Medical University,Kunming 650031,China)  
Objective To establish a validate method to get pure schwann cells with high activity.Methods The tissue plant method was employed to isolate cells,then cells were transferred to culture flask containing DMEM medium with 2.5%fetal bovine serum for inhibition of fibroblast growth.Immunocytochemistry and immunofluorescence were used to identify schwann cells with anti-S-100 antibody.Results Schwann cells with high activity were harvested,the purity of the isolated cell proportion was more than 98%.Conclusion This method can get almost pure schwann cells.It is worthy of further promotion.The method to purify Schwann cells with high activity is established and should be recommended for a wider use.
【Fund】: 国家自然科学基金资助项目(81060141);; 湖北省自然科学基金资助项目(2011CDB128)
【CateGory Index】: R329
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1 WANG Shiqing,FAN Zhihai,SHEN Yixin.Department of Orthopedics,the Second Affiliated Hospital,Soochow University,Suzhou Jiangsu,215004,P.R.China.;STUDY ON ISOLATION AND PURIFICATION OF PRIMARY SCHWANN CELLS FROM DIFFERENT PARTS OF NERVE TISSUE IN RATS[J];Chinese Journal of Reparative and Reconstructive Surgery;2011-02
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