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Method on Toluidine Blue Staining for Rapid Presenting Mast Cells

Wu Min Hu Tianhan Zhou Hongming Yao Jianshe(Morphologic Experimental Center,Wannan Medical College,Wuhu,241002,China)  
Objective To make the cytoplasm of particles in the mast cells keep from being dissolved by water soluble liquid in the process of preparing specimens,and to make the color of particle in the specimen appear metachromatic.Methods The rats weighted more than 20g were selected,and specimen were performed by one step specimens fixed with the dyeing operation,using 70% ethanol solution as the toluidine blue dye solvent.Results Mast cells displayed a complete outline contrasting underlay background,and purple intracytoplasmic basophilic bulky particles appeared clear and colorful.Conclusion This method simplify operating procedures,keep the mast cell granules well,and make particulates metachromatic.So it is very suitable for making mast cells teaching specimens.
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