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《Sichuan Journal of Anatomy》 2015-01
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The Gene Expression of Bcl-2 and Bax in Spinal Cord Contusion Rat Deteced by Real-time PCR

Shi Lanlan;Huangting;Luo Ji;Zhong Zhanqiong;Xia Qingjie;Zhang Xiao;Wang Tinghua;Experiment Technology Center of Preclinical Medicine,Chengdu Medical College;Institute of Neurological Disease and Translation Neuroscience Center,West China Hospital of Sichuan University;  
Objective This study aimed to investigate Bcl-2and Bax gene expression change at different time points following contusive spinal cord injury.Methods A total of 72 adult female SD rats were randomly divided into nine groups:sham group;spinal cord contusion 6h,12 h,1d,3d,5d,7d,14 dand 28dgroups.Real-time PCR was used to investigate the Bcl-2and Bax gene at 6h,12 h,1d,3d,5d,7d,14 dand 28dafter injury.Results Real-time PCR found that the mRNA levels of Bcl-2and Bax in sham group were high,however,they both decreased immediately after injury.At 6hfollowing injury,the expression of Bax gene was significantly decreased(P0.01)when compared with sham group.While Bax gene level was then increased at 12 hand the difference between 6hgroup and 12 hgroup was statistically sigificant.Moreover,an increasing expression of Bax was discovered in 3dgroup(P0.01)and it contained a similar level until 28 dafter injury.On the other hand,Bcl-2gene significanly decreased in spinal cord contusion 6hgroup compared with sham group(P0.01),and immediately increased at 12 hafter injury(P0.01),then decresed again at 1d(P0.01).Untill at 14 dfollowing injury the expression of Bcl-2gene was significantly increased(P0.01).Conclusion After spinal cord injury,the gene expression of Bcl-2and Bax were indeed changed and these suggested that Bcl-2and Bax gene expression change involved in the mechanism of spinal cord contusion.
【CateGory Index】: R651.2
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