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《Sichuan Journal of Anatomy》 2015-01
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A Feasibility Study Using Primary Antibodies from the Same Species in Immunofluorescence Double Staining

Chen Xi;Liu Zhe;Deng Maolin;Feng Shi;Department of Gastroenterology,Affiliated Hospital of Chengdu University;  
Objective To discuss the feasibility of using primary antibodies from the same species in immunofluorescence double staining.Methods IL-17 and GFR-α1(the subset of the specific receptor of glial cell line-derived neurotrophic factor,GDNF)in mice colon tissue were detected by sequential immunofluorescence double staining.Results Both red and green fluorescence were found in some cells in the colonic mucosa and submucosa of mice with colitis,suggesting that GFR-α1is expressed in IL-17+ Th17 cells.Conclusion The first antibodies from same species can be used with sequential method of immunofluorescence double staining when the target antigens are expressed in different locations.
【CateGory Index】: R446.6
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 LU Ju,SUN Wei,CHEN De-ying (Central Laboratory,College of High Altitude Military Medicine,Third Military Medical University,Chongqing 400038,China);Preparation of immunofluorescence double staining sample for laser confocal microscope[J];Immunological Journal;2007-03
【Co-citations】
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2 ZHUANG Xiang-sheng, ZHANG De-ming, ZHOU Lun-jiang, WEI Hong, WANG Long-bai, XU Li-hua, LU Yin-hua, CHEN Pu-yan (Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of AgricuLture Sciences, Fuzhou, Fujian 350013, China; College of Animal Medicine, Nanjing Agriculture University, Nanjing, Jiangsu 210095, China);Pathogenic investigation of infectious respiratory syndrome occurred in swine herds in Fujian Province[J];Fujian Journal of Agricultural Sciences;2004-01
3 Xian-Ning Liu1, Xiu-Ping Zhu1, Jie Wu21Shaanxi Provincial Institution of Ophthalmology, Xi'an Eye Hospital, Xi'an 710002, Shaanxi Province, China; 2Department of Ophthalmology, the First Hospital of Xi'an City, Xi'an 710002, Shaanxi Province, China;Comparison between two kinds of laboratory diagnosis methods of fungal keratohelcosis[J];International Journal of Ophthalmology;2008-07
4 WANG Li qiang LU Zu kang LIN Bin (CNERC for Optical Instrument, Zhejiang University,Hangzhou 310027,China);Biochip and fluorescence detection[J];Optical Instruments;2002-Z1
5 Ni Wei;Shi Weiqun;Tang Cuilan;The Second Affiliated Hospital of Zhejiang Chinese Medical University;;Effect of Qi Ling Mixture on Lamivudine Therapy on Th17 Cells and Treg Cells in CHB Patients[J];Journal of Zhejiang Chinese Medical University;2014-10
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9 Chen Song, Ge Jinwen (Laboratory of Vascular Biology, TCM University of Hunan, Changsha, 410007)[BHDWG3mm,WK40W] 〔;Antagonistic Effect of Naotaifang Extract on the Apoptosis of Human Umbilical Cord Endothelial Cell Induced by rhTNF-α[J];Journal of Hunan Traditional Chinese Medicine University of Hunan;2003-04
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China Proceedings of conference Full-text Database 5 Hits
1 ZHOU Liang~1,LEI Lian-cheng~1,XIE Fang~1,LI Shu-qing~2,LI Lin-xi~1,HAN Wenyu~(1*) (1.College of Animal Science and Veterinary Medicine,Jilin University,Changchun 130062,China; 2.Shang Hai Entry-Exit Inspection and Quarantine Bureau,Shanghai 200135,China);Expression and analysis of adhesive activity of functional domain of Trimeric autotransporter adhesins in A.pleuropneumoniae[A];[C];2010
2 LIU Yingnan~1,ZHANG Yucheng~1,DU zhenwu~1,WU Mei~1, LI Xiuying~1,YANG Qiwei~1,BAI Jinping~2,ZHANG Guizhen~1 (1.Research Center,China-Japan Union Hospital of Jilin University,Changchun,130031 2.Department of pathology,School of Basic Medical Sciences,Jilin University,Changchun 130021,China);Applied research of laser scanning confocal microscopy[A];[C];2013
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