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《Sichuan Journal of Anatomy》 2015-01
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A Feasibility Study Using Primary Antibodies from the Same Species in Immunofluorescence Double Staining

Chen Xi;Liu Zhe;Deng Maolin;Feng Shi;Department of Gastroenterology,Affiliated Hospital of Chengdu University;  
Objective To discuss the feasibility of using primary antibodies from the same species in immunofluorescence double staining.Methods IL-17 and GFR-α1(the subset of the specific receptor of glial cell line-derived neurotrophic factor,GDNF)in mice colon tissue were detected by sequential immunofluorescence double staining.Results Both red and green fluorescence were found in some cells in the colonic mucosa and submucosa of mice with colitis,suggesting that GFR-α1is expressed in IL-17+ Th17 cells.Conclusion The first antibodies from same species can be used with sequential method of immunofluorescence double staining when the target antigens are expressed in different locations.
【CateGory Index】: R446.6
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