EFFECTS OF ULTRASOUND ON SPERMATOGENIC EPITHELIA IN MICE
Xue Qingshan; Yuan Shiqu(Department of Histology and Embryology, Sun Yat-Sen University of Medical Sciences,Guangzhou 510098)(Department of Biology, Shanxi Normal University, Xi'an 710062)
The testes in scrota of mice were irradiated by ultrasound with a power of 5 W/cm2 and a frequency of 1. 10 MHz for 5 minutes(experiment gorupⅠ) and 10 minutes (experiment group Ⅱ) separately. Histological sections of the animal testes of the experimental groups were prepared after 24 hrs, 48 hrs, and 7 days of the ultrasound treatment respectively.Light microscopic structures were observed and compared between the control and the experimental groups. Results displayed that : (1) Seminiferous tubules from the ultrasound treated testes atrophied with the tubule diameter shrunk; Spermatogenic epithelia of the experimental animals got thinner with the spermatogenic stages absent; Spermatogenic cell numbers reduced with no spermatozoon developed. (2) Ultrasound in the present dose did more serious damages to the spermatogenic epithelia when applied for 10 minutes than that for 5minutes. (3) From 24 hrs to 48 hrs after the ultrasound treatment, damages to the spermatogenic epithelia became more significant. After 7 days of the irradiation, the histological structures of the spermatogenic epithelia showed some recoveries. No sperm developed. (4)Spermatogenic cells affected by the ultrasound contained mainly spermatocytes , spermatids,and spermatozoa. However, spermatogonia and the Sertoli cells manifested no obviously microscopic structure changes. All the above- mentioned results indicate that ultrasound could suppress the spermatogenesis in mice, and that the effects may be reversible.