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《Journal of Shandong Agricultural University》 2004-04
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STUDIES ON POLYMORPHISMS OF MITOCHONDRIAL CYTOCHROME B GENE SEQUENCES OF MAIN NATIVE SHEEP BREEDS IN SHANDONG

ZHANG Chuan-sheng1,GENG Li-ying1, DU Li-xin2(1.College of Life Science , Shandong Agricultural University,Taian 271018, China;2.College of Animal Science and veterinary Medicine, Shandong Agricultural University,Taian 271018, China;3.Animal Husbandry Research Institution of Chinese Agricultural Science College,Beijing 100094,China)  
In this study ,fragments of mitochondrial DNA cytochrome b gene (each 415 base pairs in length ) have been characterized from Little-tailed sheep, Large-tailed sheep ,Wadi sheep and by sequencing of the recombinant plasmids ,of which 7 variable sites were identified after combining and aligning the data with the homologous sequence .And the value of average sequence variation in sheep population (π) is 1.15% .Our results suggest that the genetic diversity within the sheep is remarkably scarce and these sheep breeds may have originated from a late common ancestor. Phylogenetic trees were constructed with neighbor joining method with the Lubei White goat as outgroup. The phylogenetic relationships of mitochondrial DNA suggested that Wadi sheep be more closely related with Large-tailed sheep bread.
【Fund】: 国家自然科学基金项目 (编号 30 2 70 94 9);; 山东省自然科学基金项目 (编号 :Y97D0 30 6 2 )资助~~
【CateGory Index】: S826
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