INDUCTION AND CULTURE OF CALLI FROM MIKANIA MICRANTHA KUNTH
LIAO Jin-song,GUO Yong(Food and biological engineering college, SouthChinaUniversity of Technology, Guangzhou 510640,China)
Factors affecting induction of callus in Mikania micrantha Kunth was studied in the paper. The result showed that auxiliary shoot and bud tip has a higher level of callus induction ratio: the best callus induction medium was MS wi th the addition of 2,4-D2.0mg/L+KT1.0mg/L, pH 5.7;culture in dark under 25±1℃。While callus subculture conditions has been optimized , that is cultured with the addition of coconut juice 15%, Vc 5.0mg·L -1,2,4-D0.5mg/L+NAA2.0mg/l+KT0.5mg/L+BA0.5mg/L,and the callus of Mikania micrantha Kunth could inhibit browning effectively and good growth effect after continuous subculture.
【CateGory Index】： Q943