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《Journal of Shandong Agricultural University(Natural Science Edition)》 2007-01
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FLUORESCENT PROPRERTIES OF N~2,N_3-ETHENOGUANOSINE AND ITS INTERACTION WITH BSA

WU Chun-hui GAO Ji-gang ZHOU Jie~* CHEN Chang-bao (College of Chemistry and Material Science,Shandong Agricultural University,Taian 271018,China)  
The guanoside derivative N~2,N_3-ethenoguanosine(εG)was synthesized and its ultra and fluores- cent properties at various pH were described.The results indicate that the equilibrium amongεGH~+,εG andεG~- exist.The neutral species is the origin of fluorescence.The fluorescent properties in different media were studied.It indicates that hydrogen bonds are formed betweenεG and water,which results in fluorescence inten- sity increased.Based on the action of fluorescence quench ofεG on bovine serum albumin(BSA),The binding constant and the number of binding site were determined,which were 3.891×10~3dm~3/mol and 1,respectively. The quenching mechanism was discussed.It indicates that the powerful interaction exists betweenεG and BSA.
【Fund】: 山东省中青年奖励基金资助项目(No:02BS019)
【CateGory Index】: R96
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