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《Journal of Shandong Agricultural University(Natural Science Edition)》 2007-04
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PLASMID PROFILE ANALYSIS ON PATHOGENIC E COLI FROM CHICKENS

Zhang Li-ping1,Tian Zhao-Ju1,Zhang Xi-lei1,WANG Chun-min2,LU Feng3,Zhao Hong-kun1(1.Shandong Agricultural University,Taian 271018,China;2.Zhucheng Foreign Trade Company,Zhucheng,262200,China;3.Shandong Veterinary Station,Jinan 250000,China)  
Thirty-Two strains of Escherichia coli(E.coli) had been isolated from heart blood specimens of E.coli infected chickens in six farms in Shandong zhucheng Foreign Trade Group Corp.All of them were examined for pathogenicity in susceptible chickens inoculated.All strains of E.coli isolated from blood specimens were proved to be virulent.In the screening test of plasmid DNA from 14 strains of E.coli isolates and plasmid DNA fragments generated by restrtctton endo-nuclease Bamhi,simple agarose gel electrophoresis was employed.Results showed that most of E.coli isolates from the same infected flocks on the same farm had similar plasmid profile,and its fragments sizes were similar too,and that these isolates had common plasmids.Results!showed!that those isolates which isolated from different flocks of chicken and had different plasmids were in different serotypes,This confirmed that plasmids could be transfered in different serotypes of E.coli
【CateGory Index】: S852.61
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