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《Journal of Shandong Agricultural University(Natural Science Edition)》 2018-06
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Preparation and Specificity Identification of Polyclonal Antibodies Against Fowl Adenovirus Hexon Protein

NIU Yu-juan;SUN Qin-qin;ZHAO Jun;SUN Wei;ZHANG Hui-xia;ZHANG Gui-hua;XIAO Yi-hong;SHANG Ying-li;LIU Si-dang;College of Animal Science and Veterinary Medicine/Shandong Agricultural University;Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention;Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention;Penglai City Xiao Menjia Veterinary Station;  
The purpose of this study is to prepare polyclonal antibodies against fowl adenovirus(FAd V) Hexon protein and to identify its specificity. The recombinant prokaryotic expression plasmid p ET-28 a-Hexon was constructed and transformed into E.coli BL21(DE3), IPTG induction, the recombinant protein was identified by SDS-PAGE, and the protein was purified. The polyclonal antibodies of Hexon protein was prepared by routine immunity in New Zealand white rabbits with purified fusion protein. The specificity of polyclonal antibodies was identified by indirect immunofluorescence, immunohistochemistry and Western blot. The results showed the prokaryotic expression of Hexon protein was high and existed in the form of inclusion body, and its molecular weight was about 43 k Da;Polyclonal antibodies having specific reaction with FAd V and high potency could be obtained in the immunized rabbits. The above results indicated the polyclonal antibody of FAd V Hexon protein prepared in this study proved to be of good specificity and provided a good material basis for the diagnosis, pathogenesis study and subunit vaccine development of avian adenovirus diseases.
【Fund】: 山东省“双一流”奖补资金(2017)
【CateGory Index】: S852.65
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【Citations】
Chinese Journal Full-text Database 1 Hits
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【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 LI Hai-ying1,YIN Yan-bo1,WANG Jian-lin1,GUO Yan-yan2,WANG Xiao-hong1,WANG Shou-chun1,ZHANG Yi3,WANG Guang1,LI Ji-da1 (1.College of Animal Science and Veterinary Medicine,Qingdao Agricultural University,Qingdao,Shandong,266109,China;2.Oland-better Bionengineering Company,Qingdao,Shandong,266101,China;3.College of Veterinary Medicine,China Agriculture University,Beijing,100193,China);Pathological Observation of Chicken Inclusion Body Hepatitis[J];动物医学进展;2010-07
2 Hao Xianpu; Wang Fenglong;Gu Yufang;Lin Xi(Department of Animal Medicine,Inner Mongolia Institute of Agriculture and Animal Husbandry);Pathological Studies on Inclusion Body Nepatitis inChicken-pathological Studies on Chick Embryo Experimentally Infected with Field Virus IBHV-93b[J];内蒙古农牧学院学报;1996-03
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