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《JOURNAL OF SHANDONG AGRICULTURAL UNIVERSITY》 1996-01
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PARTIAL PURIFICATION AND CHARACTERISTICS OF POLYPHENOL OXIDASE FROM PRUNUS PERSICA L.(FEICHENG PEACH)

Feng Xin;Zhou Hongwei(Food Science Department,Shandong Agricultural University, Taian,China 271018)  
Polyphenol oxidase (PPO) has been partially purified from P. persica (Feicheng peach). At the end of the (NH4)2SO4 precipitation and dialysis step, 5.11-fold purification was achieved. The enzyme was active in catechol,pyrogallol or DL-DOPA among of which pyrogallol was the best substrate. The optimum pH for the PPO was 6.8. The PPO activity at 35℃ did not decrease in 30 min. The effects of various compounds as inhibitors of the reaction catalysed by the enzyme were tested. These results revealed that the inhibitory effects of thiourea and Na2S2O3·5H2O were very strong,the inhibition at 0. 43 mM were 80. 5% and 100% respectively
【CateGory Index】: S662.101
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