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Evaluation of a Direct Immunoinhibition Mothod for Measuring High Density Lipoprotien Cholesterol

Jiang Xingliang,Zhang Jun∥Department of Laboratory,Affiliated Hospital of North Si Chuan Medical College,Nanchong,637000  
Objective:To evaluate whether a kit of direct immunoinhibition assay for measuring high density lipoprotien cholesterol(HDL C) was suitable for clinical applied requirement or not.Methods:The immunoinhibition method was compared with PTAmethod and selective inhibition method.Moreover,Its linear range,the precision,the accuracy and the interfere factors were analyzed.Results:The regression equation of immunoinhibition method(Y) and PTA method(X 1)was Y=0.993X 1+ 0.213 , r =0.981;and inhibition method(X 2) was Y=1.01X 2+0.177, r =0.971.There were no significant difference among mean value determined by Y,X 1,X 2( P 0.05).The linear range was 0.51~2.55mmol/L.The precision of high,middle,low HDL C concentration specimen were tested,the CV in within run were 1.35%,1.67%,and 2.01%,the CV in the daytime run were 2.78%,3.14%,and 3.43%.The recovery rate were 97.8%~100.9%.The interference of bilirubin(200μmol/L),hemoglobin(5g/L),ascorbic acid(200μmol/L) and TG(10mmol/L) were insignificant.Conclusion:The kit of direct immunoinhibition assay producted by Co.Heguang,Japan for ditermination HDL C is suitable clinical appiled requirement,and it can be used in full automatic analysis.
【CateGory Index】: R446.1
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