Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Sichuan Journal of Physiological Sciences》 2007-01
Add to Favorite Get Latest Update

Cloning and eukaryotic expression of the LAg42 membrane protein gene of Leptospira interrogans serovar Lai

Liu Yu,Shang Zheng-ling,Zhong Qi,Bao Lang(Research Unit of Infection and Immunity,West China Medical Center,Sichuan University,Chengdu 610041,China)  
Objective:To construct a eukaryotic expression vector bearing lag42 gene from Leptospira(L.) Lai and transfect it intoCOS7 cells.Methods:The lag42 gene was amplified by PCR from genome of L.Lai 017 strain 56601strain and L.bilexa PatocI strain.The amplified DNA fragment was then cloned into vector pcDNA3.1A+and DNA sequence was performed subsequently.Later,the recombinant plasmid pcDNA3.1A+-lag42 was transfected intoCOS7 cells.RT-PCR was then used to check the transfection.Results:It was found that about 1100bp fragment was amplified in various virulent L.interrogans serovar Lai,but not in L.bilexa PatocI strain.There was a high homology of DNA sequence of the lag42 in various L.Lai.The recombinant plasmid was constructed triumphantly and successfully transfected into COS7 cells.Conclusion:These result suggested that lag42 gene is the conservative pathogenic mark in Leptospira,it may be associated with virulence and immunogenicity infection with pathogenic Leptospira.
【Fund】: 国家自然科学基金No.30471546资助
【CateGory Index】: R377.5
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved