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《Sichuan Journal of Physiological Sciences》 2008-01
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Inhibition of naringin on production of RANTES in HaCaT cell

Liu Liao, Hao Jie, Yang Yun-xia(Department of pharmacology, West China Medical Center, Sichuan University, Chengdu 610041)  
Objective:To determine the inhibition of naringin on tumor necrosis factor-α (TNF)-α and interferon gamma-γ (IFN-γ)induced-RANTES in cultured human keratinocyte line HaCaT.Methods: Cultured HACAT cells were treated with TNF-α and IFN-γ.With pre-incubation of naringin, expression of mRNA of RANTES was analyzed by RT-PCR.Protein of RANTES in the supernatants was determined by ELISA. Results: In normal cultured HaCaT cell, RANTES mRNA and proteins were detected, TNF-α and IFN-γ significantly enhanced the mRNA expression and protein release of RANTES. Naringin(0.5, 0.25 mmol·L-1) inhibitsed TNF-α and IFN-γ-induced production of RANTES. Conclusion: Naringin can inhibit TNF-α and IFN-γ-induced production of RANTES.
【CateGory Index】: R285.5
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