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《Sichuan Journal of Physiological Sciences》 2008-02
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Antibacterial effect of urinary bladder epithelial cells against Escherichia coli

Sui Peng-nuo,Liu Yan-xiang,Liu Zhen,Zhao Li-jun,Liu wei,Liu Xing-hai,Wang Bo-yao,Huang Ning,Wu Qi(Research Unit of Infection and Immunity,West China Medical Center,Sichuan University,Chengdu 610041)  
Objective:Epithelial cells are the first barrier to protect the host from mucosal pathogens.In this study we examined antibacterial capacities of urinary epithelial cell line T24 against Escherichia coli.Methods:E.coli K12 strain and T24 cells were suspended in 1640 medium for the experiment(approximately 100 bacteria per epithelial cell),added to eppendorf tubes and then put in CO2 incubator at 37 ℃.The growth of bacteria was detected at different time points from 15 min to 120 min by the quantitative colony counting method.Results:Antibacterial effects of T24 cells against E.coli K12 strain at 15,30 and 60 min were 19.4%,53.0%,39.2% respectively.Conclusion:The direct antibacterial activities of epithelial cells may be a host defense factor to maintain the urinary bladder in a sterile situation.
【Fund】: 国家自然科学基金(NO:30570790);; 教育部博士点基金资助
【CateGory Index】: R694
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【Co-citations】
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1 Liu Yan-xiang,Zhao Li-jun,Sui Peng-nuo,Liu Zhen,Wang Bo-yao,Huang Ning,Wu Qi(Research Unit of Infection and Immunity,West China Medical Center,Sichuan University,Chengdu 610041);Antibacterial effect of airway epithelial cells against Pseudomonas aeruginosa[J];Sichuan Journal of Physiological Sciences;2009-02
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