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An improved mice model of cecal ligation and perforation for sepsis

Guo Jia-nan;Liu Wen-yu;Zhou Dong;Tian Lin-yu;Department of Neurology,West China Hospital of Sichuan University;  
Objective:To explore how to improve traditional Cecal-ligation and puncture(CLP)procedure.Methods:A total of103 KM male mice were randomly divided into three groups:antibiotic group,no antibiotic group and sham group.Antibiotic group were treated with imipenem and cilastatin;for sham group,we only open the peritoneum and then closed without therapeutic use of antibiotics.We designed a shorter incision in a new site below xiphoid,changing the dose of antibiotics after surgery and observe the survival rate after surgery.Results:Generally,among all 103 mice accepted the surgery,the survival rate of antibiotic group,no antibiotic group and sham group were with statistically difference(P=0.026).Comparison between each groups showed that:the seven days postoperative survival rate was higher in antibiotic group and no antibiotic group(P0.001);survival rate in sham group was higher than that in no antibiotic group(P0.001).However,the survival rate between antibiotic group and sham group has no difference(P=0.331).Cronbach'sαcoefficient among 10batches' s survival rate in antibiotic group is 0.954.Conclusion:Compared with traditional CLP one,improved model has a similar survival rate and a easy standard surgical procedure with smaller incision and a reliable result.
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