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《Sichuan Journal of Physiological Sciences》 2017-01
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Effects of dexmedetomidine on ROS and apoptosis in neonatal rat cardiac muscle cells

Cao Lu;Zhou Dan;Li Tao;Chen Lin-lin;Tan Xiao-qiu;Liu Li;Liu Xue-ru;Department of Anesthesiology,Affiliated Hospital of Southwest Medical University;Key Lab of Medical Electrophysiology,Ministry of Education;Institute of Cardiovascular Medicine,Southwest Medical University;  
Objective:To investigate the effects of dexmedetomidine(DEX)on ROS and apoptosis in neonatal rat cardiac muscle cells.Methods:Neonatal rats(3-4days)cardiomyocytes(NRCMs)were cultured for 24 hand divided into four groups:control group(Group C),H_2O_2group(final concentration of 500μM,Group H),dexmedetomidine group(5μM DEX,group D),and H_2O_2+DEX group(500μM H_2O_2+ 5μM DEX,group DH).Flow cytometry(FCM)was used to measure the effect of DEX on the ROS level and apoptosis of myocardial cells in rats.The level of Caspase-3and Caspase-9were detected by ELISA.Results:DEX significantly inhibited the increase of ROS levels in NRCMs induced by H_2O_2(P0.05).The increased level of Caspase-3,Caspase-9induced by H_2O_2 was significantly inhibited by DEX(P0.01 or P0.05).DEX significantly attenuated cells ratio of apoptosis induced by H_2O_2(P0.05).Conclusion:Dexmedetomidine inhibited the ROS levels and the activity of mitochondria mediated Caspase-3and Caspase-9expression and attenuated H_2O_2-induced apoptosis in myocardial cells,which may involved in the myocardial protective effect of DEX.
【Fund】: 国家自然科学基金资助项目(编号:81401632);; 西南医科大学青年基金项目(编号:2014QN-003)
【CateGory Index】: R614
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