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《Sichuan Animal & Veterinary Sciences》 2018-02
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Cloning and Bioinformatics Analysis on the TK Gene of DPV′s Virulent and Vaccine Strain

Ren Pan;Liu Qing;Li Wenwen;College of Life Science and Technology,Southwest Minzu Nationalities;  
According to the sequence of TK gene in Gen Bank,a pair of primers was designed to amplify this gene in virulent and vaccine duck plague virus strains. The purpose amplified fragment was cloned into p MD 19-T vector. The recombinant expression vector was selected and identified by PCR and restriction enzyme digestion(Bam HⅠ+HindⅢ). The positive recombinant plasmid was then sequenced and the biological information was also analyzed. The results showed this research successfully cloned the DPV TK gene,and both of the fragments were 1 077 bp,the result of bioinformatics analysis revealed the homology of two fragments up to 100%.
【Fund】: 国家农业科技成果转化资金项目——国家科技部农业项目“规模化养鸭场标准化健康养殖技术示范与推广”(2013GB2F000422)
【CateGory Index】: S852.65
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