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《Journal of Sichuan of Traditional Chinese Medicine》 2006-01
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The Impromed Method of Serum proteomics in the study of Syndrome Type in TCM

Zhong Xiaolan~1 Lu Zhiping~1 Qian Lingjia~2 Gong Jingbo~2.∥1.Nan Fang Southern Medical University(Guangzhou Guangdong 510515) 2.The Institute of Hygiene Environmental Medicine(Tianjin 300050)  
Objective:To establish and optimize a two-dimensional polyacrylamide gel electrophoresis(2-DE) the separation technique for serum proteomics,which will be used in the study of TCM Syndrome Types.Methods:Modification on serum sample administration,IPG strip,Loaded quantity and IEF parameter based on the conventional 2-DE separation,and exploration on a new mothod.Results:A steady 2-DE technique for serum proteomics was established and on ideal outcome was gained when it was applied in the study of "depressed liver" type.Conclusions:The modified method is stable,repeatable and will better base the study of serum proteomics and TCM Syndrome Types.
【Fund】: 国家自然科学基金资助项目(30371713)
【CateGory Index】: R2-03
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