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Study the effects and mechanisms of Panaxadiol Saponins on membrane stability of renal tubular cells injured by the serum of renal ischemic reperfusion

Pan Qiang-wen~1 Ran Bing~1 Guo Yong~1 Wang Qiong~2 Feng Zhi-qiang~1.1.Laboratory of Renal Function of Luzhou Medical College(Luzhou Sichuan 646000) 2.The center Laboratory of Luzhou Medical College(Luzhou Sichuan 646000)  
Objective:To observe the effects of Panaxadiol Saponins(PDS) on the membrane stability of cultured renal tubular cells injured by the serum of renal ischemic reperfusion(IR),and discuss their mechanisms.Methods:Firstly,acquire the serum of rabbits with renal IR(SIR) and the control serum of rabbits(SC),to take the different culture mediums(common culture mediums?SIR?SC and SIR+PDS)in diverse groups,and culture renal tubular cells for 96 hours,then,dete of the content of alondialdehyde (MDA) and the activity of Superoxide Dismute(SOD),lactate dehydrogenase(LDH),ATPase in the culture medium or cells by biochemistry method.Results:The content of MDA in the culture medium,showing the degree of lipid Superoxide reaction,and LDH in the culture medium,indicating the integrality of plasmalemma,were significantly increased,but the activity of SOD,Na~+-K~+-ATPase and Ca~(2+)-ATPase in cells were remarkably decreased after treated by SIR.However,these effects of SIR could be inhibited by PDS.Conclusions:The membrane stability of cultured renal tubular cells injured by the serum of renal IR could be promoted by Panaxadiol Saponins.The possible mechanisms could be concluded as follows increasing the activity of SOD,and relieveing the injury of membrane resulting from the attacking of Oxygen free radical,increasing the activity of ATPase and maintaining the balance of the content of water and electrolyte inside and outside the cells.
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