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《Journal of Sichuan of Traditional Chinese Medicine》 2006-07
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Effect of different extracting solutions of Hirudo on bovine lens epithelial cells cultivated in vitro

Zhou Chunyang Zheng Yanlin Shen Rong Zhou Huaxiang∥Department of ophthalmology,Teaching Hospital of Chengdu University of Traditional Chinese Medicine(Chengdu Sichuan 610075,China)  
Objective:To observe the effect of different extracting solutions of Hirudo on the proliferation of bovine lens epithelial cells(LEC)cultivated in vitro,for comparing the strength of effect of different extracting solutions.Methods:Five kinds of conventional extracting solution of Hirudo(alcohol and water extracting,alcohol refluxing,water extracting,water extracting-alcohol precipitating,percolation filtration)were used in this study.Bovine lens anterior capsule was cultured in vitro with explant culture method.The passage LEC was incubated for 48h,then exposed the cells to different extracting solution in different concentrations for 24h and 72h.MTT method was used to detect the OD values and ID_ 50 was calculated.Results:There was significant difference of 24hID_ 50 as the percolation filtration group compared with the alcohol and water extracting group and the alcohol refluxing group(P0.05),and there was significant difference of 24hID_ 50 as the alcohol and water extracting solution group compared with the alcohol refluxing group also(P0.05).Conclusions:Five kinds of conventional extracting solution of Hirudo all can inhibit the proliferation of LEC cultivated in vitro,and inhibition of water extracting-alcohol precipitating solution and percolation filtration solution was more strong among them.
【CateGory Index】: R285.5
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