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Activate Effect of Tongluoshenggu Capsule on Estrogen Receptors

Wang Haibin1 Wang Junjian2 Huang Hui2 Liu Lin2 Liu Shaojun1 Xue Chanyi1 He Wei1 Fang Yueguang1 Huang Zhiwei21.Department of Orthopedic Surgery,First Affiliated Hospital of Guangzhou University of TCM & Pharmacology(Guangzhou Guangdong 510405,China) 2.Guangzhou Institute of Biomedicine and Health,Chinese Academy of Sciences(Guangzhou Guangdong 510663,China)  
Objective:The establishment of Ga14DBD-ERα or ERβ LBD reporter system to study the effect of Tongluoshenggu Capsule on ERs.Methods:Gene reporter system was generated by amplifying the ligand binding domain(LBD)with Pfu polymerase and subcloning the product into the eukaryotic expression vector pCMV-BD,This system was employed to study the relation between Tongluoshenggu Capsule and ERs.Cell activity were detected by MTT.Results:Gene reporter system using ER-LBD is established,Tongluoshenggu Capsule can activate ERs and improve cell activation.Conclusions:The mechanisms of Tongluoshenggu Capsule maybe related to its effect of activating ERs,Gene reporter system can be used to study the mechanisms of Chinese Traditional Medicine.
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