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Bone Proteomic Analysis About Chinese Medicine Action On Rat Ovariectomy Model of Osteoporosis.

Wang Haibin~1 Liu Jianren~1 Fan Yueguang~1 Xion Lihua~1 He Wei~1 Peng Xuanxian~1 Wang Sanying~1.1.Department of Orthopedic Surgery,First Affiliated Hospital of Guangzhou University of TCM(Guangzhou Guangdong 510405,China) 2.Center for proteomics,Department of Biology,School of Life Sciences,Xiamen University(Xiamen Fujian 361005,China)  
Objective:To study the mechanism about osteoporosis of OVX(ovariectomy) and Chinese Medicine action on osteoporosis in proteomics,it is of benefit to prevent and cure this disease.Methods:Bilateal OVX in rats was performed as osteoporosis model.Rat was randomly divided into three groups:control group,osteoporosis model group and Chinese Medicine therapy group.The pathology of bone was examined after 6 weeks.In the present proteomic study,we characterized the protential effects of OVX and Chinese Medicine on protein expression in rat bones.Using two-dimensional gel electrophoresis,mass spectrometry,and Matrix science Ltd database,we elementarily identified three variational proteins.Conclusions:Three proteins were identified as proteins similar to thioredoxin peroxidase 1,Myosin light polypeptide 2 and ubiquitin-conjugating enzyme E2-17kD.These proteins have been demonstrated to be postmenopausal proteins.These results can provide valuable experimental evidences for the research for the molecular mechanism of osteoporosis which was response to OVX and Chinese Medicine in bone.
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