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《Journal of Sichuan of Traditional Chinese Medicine》 2011-01
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Content Determination of Orientin in Macro-porous Resin Extract of Fenugreek Seeds

Wu Yan Yuan Chongjun Yang Hong Luo Sen Chen Shuai Xu Xue-min Sichuan Academy of Chinese Medicine Science(Sichuan Chengdu 610041,China)  
To determine the content of orientin in macroporous resin extract of fenugreek seeds.Methods:It was determined with HPLC.The column was ODS C18 column(150mm×4.6mm,5μm).The mobile phase was acetonitrile-0.2% H2PO4(15∶85).The wavelength was 350nm.Results:The standard curve was linear in the range of 0.023~0.279μg(r=0.9999)for orientin.The average recovery of orientin were 100.1%.The contents of orientin from 3 simples were 1.30%、1.41%、1.34%.Conclusion:The method is simple,quick and accurate.It is suitable for content determination of orientin maroporous in resin extract of fenugreek seeds.
【CateGory Index】: R284.2
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