Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《JOURNAL OF MOUNTAIN AGRICULTURE AND BIOLOGY》 2000-03
Add to Favorite Get Latest Update

Preparation and detection of egg drop syndrome virus DNA probe

HAN Li zhen 1,CAO Wei 2,WU Tong 1(1 Department of Animal Science, College of Biotechnology, Guizhou University, Guiyang 550025,China;2 Guiyang Jingzhu University, Guiyang 550001,China)  
The Digoxigenin(Dig) probe with HS-1 whole genomic DNA was developed by purified EDS virus. In the hybridization, the probe reacted only with 3 strains of EDS virus, but not with Marek's disease virus, normal duck embryo allantoic fluid DNA samples. So it was of high specificity and sensitivity and 30 pg DNA could be detected in experiment. By Dot-bolt hybridization the probe had detected virus's DNA in cloacal swabs for early diagnosis, and the positive rate of samples was 88.89% in artificial infected hens. In addition it could be used in EDS recessive infection.
【Fund】: 贵州大学校基金资助项目
【CateGory Index】: S858.31(73);;S85
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 1 Hits
1 FANG Hao1,SONG Nian-hua2,YAO Zhi-wei1,XIONG Yuan-lin1,WANG Song-tai1(1.Department of Bioengineering,Wuhan Bioengineering Institute,Wuhan 430415,China;2.Hubei Diagnosis Center of Animal Disease,Wuhan 430064,China);Preparation of the Probes Used for H5 and H9 Subtype of Avian Influenza Virus[J];Hubei Agricultural Sciences;2007-02
【Citations】
Chinese Journal Full-text Database 5 Hits
1 Zhang Zijun Hu Muzhi (Gongbei Animal & Plant Quarantine Bureau,Zhuhai Guangdong,519020);STUDY ON DETECTION OF EGG DROP SYNDROME(EDS-76)VIRUS USING BIOTINYLATED RECOMMBINANTPLASMID PROBE[J];CHINESE JOURNAL OF VIROLOGY;1995-02
2 Cao Hong-jin; Wang Wen-zhi; Xu Xue-meng; DU Yu-qing(Institute of Animal Husbandry & Veterinary Medicine,Shandong Academy of Agricaltural Sciences)Gong Yun-hao;Sun Shu-fang(Animal) Quarantine Institute Ministry of Agriculture);Preparation of DNA probe for egg-drop syndromes (EDS-76) Virus[J];Chinese Journal of Animal Quaranitine;1994-05
3 Wang XueminZheng Mingqiu et al(Harbin Agricultural College)(Nanjing Agricultural University);Detection of Egg Drop Syndrome Virus by DotELISA[J];;1998-01
4 Sun Gang,Pan XingguangLiu Shanggao(Heilongjiang Veterinary Sanitation and Antiepidemic Station,Harbin 150030)(China Agricultural University,Beijing 100094);Deteetion of EDS76 Virus Nuvleic Acid by Digoxigenin labeled DNA Probe[J];;1998-01
5 Duan Yuyou; Cui Zhizhong; Gao Song; Ge Tiantong(Fac of Anim Sci and Vet Med,Agri Coll of Yangzhou Uni ,Yangzhou 225001);Preparation and Application of DNA Probe of Chicken Egg Drop-syndrom Virus[J];CHINESE JOURNAL OF VETERINARY SCIENCE;1995-03
【Co-references】
Chinese Journal Full-text Database 7 Hits
1 HUANG Guang-ming, ZHANG Man-fu, QIAO Su-lan (Department of Molecular Virology, College of Biological Science, China Agricultural University, Beijing 100094, China);Study on the Early Infecting Process of Infectious Bursal Disease Virus in Chicken by in situ RT-PCR[J];Chinese Journal of Virology;2001-01
2 Tian Zhigang; Sun Rui; Liu Jie; Li Baiging;Zhang Jie; Zhang Jianhua(Cancer Biotherapy Center of Shandong Academy ofMedical Sciences (Jinan 250001));DETECTION OF HUMAN IL-6 GENE EXPRESSION USING IL-6 cDNA PROBE[J];SHANGHAI J.OF IMMUNOLOGY;1994-03
3 Cao XieZheng,Ke ZhongZhe,Zhang LiHuai(Jianjin Exit and Entry Inspection and Quarantine Service,Tianjin,300456);Establishment of Positive Threshold Value of Microtitre HI Test for Eggs Drop Syndrome (EDS-76)[J];China Animal Quarantine;2003-01
4 Sun Gang,Pan XingguangLiu Shanggao(Heilongjiang Veterinary Sanitation and Antiepidemic Station,Harbin 150030)(China Agricultural University,Beijing 100094);Deteetion of EDS76 Virus Nuvleic Acid by Digoxigenin labeled DNA Probe[J];;1998-01
5 Li Ting,Zhang Po,Huang Feng,et al(Department of Pathology & School of Health);PREPARATION OF MDM_2 ON COGENE cDNA PROBE AND ITS DETECTION IN TUMOR TISSUES[J];JOURNAL OF CHINA-JAPAN FRIENDSHIP HOSPITAL;1995-01
6 REN Xiu-lian,ZHUANG Guang-lun,XU Yan-wen,ZHOU Can-quan,FANG Cong,ZHANG Min-fang(Reproductive Medical Center,The First Affiliated Hospital,SUN Yat-sen University,Guangzhou 510080,China);Application of Whole Chromosome Painting Probes in Fluorescence In-situ Hybridization of First Polar Bodies[J];Journal of Sun Yat-Sen University(Medical Sciences);2006-01
7 ZHANG Wan-po1,CHENG Guo-fu 1* ,GU Chang-qin1,LI De-xue2,SONG Nian-hua2,BI Ding-reng1, HU Xue-ying1,HU Si-shun1,HU Zhi-bin2 (1.College of Veterinary Medicine,Huazhong Agricultural University,Wuhan 430070,China;2.Hubei Diagnosis Center of Animal Disease,Wuhan 430064,China);Detection of H9N2 Subtype Avian Influenza Virus by in Situ Hybridization[J];Chinese Journal of Veterinary;2005-04
【Secondary References】
Chinese Journal Full-text Database 3 Hits
1 LU Cui,MA Xiao-ming,YIN Yan-bo,SHAN Hu(Faculty of Animal Science and Veterinary Medicine,Qingdao Agricultural University,Qingdao 266109,China);Preparation and Application of M Gene Nucleic Acid Probe of Swine Influenza Virus[J];Acta Agriculturae Boreali-Sinica;2009-01
2 ZHONG Ming DONG Zhang-cheng GUO Zhi-fu MA Hui CHEN Li-jing ZHANG Li (Key Lab.of Agric.Biotechnol.of Liaoning Prov.,Shenyang Agric.Univ.Shenyang 110161);Conservative Sequence Cloning and Probe Preparation for α Sub-Radical of Dioxygenase[J];Journal of Microbiology;2008-03
3 Lü Cui,MA Xiaoming,YIN Yanbo,WEN Jianxin and SHAN Hu*(Faculty of Animal Science and Veterinary Medicine,Qingdao Agricultural University,Qingdao Shandong 266109,China);Preparation and Application of HA Gene Nucleic Acid Probe of H3 Subtybe Swine Influenza Virus[J];Acta Agriculturae Boreali-Occidentalis Sinica;2009-03
【Secondary Citations】
Chinese Journal Full-text Database 6 Hits
1 Sun Yi Chen Boquan (Institute of Virology, Chinese Academy of Preventive Medicine, Beijing);PREPARATION OF MONOCLONAL ANTIBODIES AGAINST RABIES VIRUS AND ANALYSIS OF ANTIGENIC EPITOPES OF RABIES VIRUS GLYCOPROTEIN[J];Chinese Journal of Virology;1990-02
2 ZhaoYufangYangZengqiZhangShuxiaHeHongquanWuLianfuKangZuowen(ResearchLaboratoryofPoultryDisease,NorthwesternAgriculturalUniversity,Yangling,Shaanxi712100);InvestigationofEggDropSyndromeofChickensinGuanzhongAreaofShaanxiProvince[J];ACTA UNIVERSITATIS AGRICULTURAE BOREALI-OCCIDENTALIS;1996-03
3 Zheng Shijun, Gan Menghou ( Veterinary College, Beijing Agricultural University );STUDIES OF INDIRECT DOT-ELISA FOR THE DETECTION OF NEWCASTLE DISEASE VIRUS[J];Chinese Journal of Animal and Veterinary Sciences;1992-02
4 Duan Yuyou; Cui Zhizhong; Gao Song; Ge Tiantong(Fac of Anim Sci and Vet Med,Agri Coll of Yangzhou Uni ,Yangzhou 225001);Preparation and Application of DNA Probe of Chicken Egg Drop-syndrom Virus[J];CHINESE JOURNAL OF VETERINARY SCIENCE;1995-03
5 Wang Zelin;Cheng Hongying;Wang Li(Henan Agricultural University,450002);Establishment of a Sandwich Using Monoconal Antibody ELISA Test for the Detection of EDS- 76 Virus[J];CHINESE JOURNAL OF VETERINARY MEDICINE;1994-11
6 Cheng Anchun Wang Minshu Liao Dehui Chen Xiaoyue Zhong Nina (College of Animal Science and Technology,Sichuan Agricultural University Yaan,Sichuan,China 625014);Studies on the Detection of Egg drop Syndrome Virus Using Silver enhanced Colloidal Gold Probe Assay[J];CHINESE JOURNAL OF VETERINARY MEDICINE;1997-07
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved