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Effects of heavy metal ions stress on reactive oxygen species in the creeping bent seedlings leaves

WANG Hui-zhong~(1,2),ZHANG Xin-quan~2,HE Cui-ping~3(1.College of Life Science,Guizhou University,Guizhou Guiyang 550025,China;2.College of Animal Science,Sichuan Agricultural University,Sichuan Yaan 625014, China;3.Agricultural College,Guizhou University,Guizhou Guiyang 550025,China)  
By using fluorescence spectrophotometry method,a systematic study was conducted to determine the effect of cadmium and lead ion stress on reactive oxygen species(ROS) in creeping bent Agrotis stolonifera leaf tissue.All experimental numerical values were analyzed by using the ANOVA of the software SPSS 13.0.The results showed that the(antimycin) A and heavy metal ions stress could significantly effect the opposite contents of hydrogen peroxide(H_2O_2) in leaf tissue.The antimycin A,a coenzyme Q depressor at electronic pipeline in mitochondria,and heavy metal ions could prevail on enhancing of dichlorofluorescein(DCF) fluorescent value.This means that heavy metal ions indeed prevailed on producing ROS in plant cells.
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