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Study on vegetative compatibility among isolates of Paecilomyces cicadae(Miquel)Samson

ZHAO Jie-hong,LIU Ai-ying~(**),LIANG Zong-qi(Institute of Fungus Resources,Guizhou University,Guizhou Guiyang 550025,China)  
Thirty-two single spore isolates were obtained from seven strains of Paecilomyces cicadae(Miquel)Samson that were collected from Yunnan and Guizhou provinces.On KPS containing 20g·L~(-1) KClO_3,several sectors appeared from different directions of one clone after incubating at 25℃ for five days.These sectors could be divided into three types.When 110 sectors were selected and inoculated on minimal medium,we obtained sixty nitrate nonutilizing(nit)mutants from them as compared with their morphology of clones.It showed that there was no special corresponding relation(between nit mutants) and sector types.When Vegetative compatibility tests were carried out by pairing the sixty nit mutants on minimal medium at 25℃ for twenty-one days,only seven nit mutants collected from Mojiang of Yunnan province were vegetatively compatible while others showed negative reaction.The hypha in mating line stained by Hoechest 33258 showed that hypha with two or more nucleus and phenomenon of"H" anastomosis were observed.But we didn't find any conidia with two or more nuclei.
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