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Development of diagnostic techniques of foot and mouth disease

XIAN Si-mei~1,ZHOU Bi-jun~(1*),WEN Ming~1,FANG Ying~2,WANG Chen~2(1.College of Animal Science,Guizhou University,Guizhou Guiyang 550025,China;2.Agricultural Bureau of Guiyang City,Guizhou Guiyang 550004,China)  
The development of biological test,diagnostic techniques of serology and molecular biology for foot and mouth disease(FMD) was summarized in this paper.In the aspect of biological test,the diagnosis of FMD was mainly depended on animal experiment and cell culture.The diagnostic techniques of serology included complement fixation test,virus neutralization test,agglutination test,immunodiffusion,enzyme-linked immunosorbent assay(ELISA) and so on.Along with the development of molecular biology,the diagnosis of FMD has advanced to molecular level from cytological level,nucleic acid hybridization and RT-PCR diagnosis of FMD virus have been established.These techniques have higher sensitivity and specificity compared with the traditional methods.
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