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《Journal of Mountain Agriculture and Biology》 2018-06
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Identification of 30 Treespeciesin Guizhou Province by DNA Barcoding Based on nrITS

CHENG Yu;CAI Sha-sha;SHU Jian-feng;YUAN Hao;WU Ying;TU Cui;YANG Peng;ZHAO Cai;College of Life Sciences,Guizhou University;  
In this paper,the ITS sequences of 30 tree species of 26 genera in 19 family of Guizhou Province were amplified and sequenced by PCR. The sequence information was analyzed by MEGA 5.0,the genetic distance within and between species was calculated by the nearest distance method,and the phylogenetic tree was constructed by the adjacency method.The results showed that the length of DNA sequencesfromthe tested species were 506-684 bp,the average content of G+C was 64%,the average intraspecies genetic distance was0. 013,and average interspecies genetic distance was 0. 236. The clustering tree based on ITS sequence showed that the success rate of identifying the 30 tree species was 83%.Therefore,the ITS sequence is recommended as a candidate sequence for DNA barcodes of tree species.
【Fund】: 贵州大学博士基金(201354);; 贵州省烟草专卖局项目(201401);; 贵州省科学技术基金(黔科合J字[2014]2064号);; 大学生创新创业训练计划项目(贵大省创字[2015]004;贵大校创字[2015]024);; 贵州省中药材现代产业技术体系建设(GZCYTX-02)
【CateGory Index】: S718.4
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