The Study on Transfection of rhbFGF into Human Skin Fibroblast
DONG Zhi-ning,SUN Fen-yong,PAN Qiu-hui,HONG An(Biotechnological Institute of Jinan University, Guangzhou 510632 China)
The rhbFGF gene was cloned into the eukaryotic expression vector pEGFP-N1, just before the enhanced green flurecent protein (EGFP) gene. At the 5′ end of the fused pEGFP-rhbFGF gene, the signal peptide sequence of IL-4 was introduced in order that the pEGFP-rhbFGF fusion protein could be secreted into the medium. Liposome was used in the transfection. The expression of the fused gene was detected through fluorescent microscope and Western blot, the influence of rhbFGF gene on cell growth was observed under microscope. Results showed that the pEGFP-rhbFGF plasmid was constructed successfully and transfected efficiently into the human dermal fibroblasts with Liposome. The transfected fibroblasts can secret active rhbFGF outside of the cells and effectively stimulate the cell growth. bFGF transfection can steadly promote the proliferation of human fibroblasts.