Characteristics and Observing Method of Microvessels of Chorioallantoic Membrane in Chicken Embryo
WANG Shi-jun 1,2,SUN Jing 1,ZHANG Dong 1,GUO Ji-long 1,JIAN Long-lei 1,LIU Kai 1(1. Shandong University of Traditional Chinese Medicine,Jinan 250014;2. Nanjing University of Tradifional Chinese Medicine,Nanjing 210096)
To investigate the characteristics of microvessels of chick chorioallantoic membrane (CAM) and the method for observing the microvessels. Chicken embryo of Hailan breed was cultured. The microvessels of CAM in different cultured day were observed in normal condition and after the process of fake drug application. Results showed that the number and area of microvessels of CAM increased as the cultured day getting longer, and the vitality also increased. The microvessels of CAM cultured for five days are suitable for the study of focal drug application.