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《Journal of Biomedical Engineering Research》 2007-01
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Establishment of a Two-dimensional Electrophoresis Technologic Platform on Hepatopancreas of Penaeus chinensis

QIN Zhao-yu1,LIU Shi-lian2,SONG Chun-hua1,Zhang Xu-hua2(1.Biology Science Department in Marine College,Shandong University at Weihai,Weihai 264209,China;2.Institute of Biochemistry and Molecular Biology,School of Medicine,Shandong University,Jinan 250012,China)  
To establish a two-dimensional electrophoresis technology platform on Penaeus chinensis.Hepatopancreases of Penaeus chinensis were taken to establish a technical system by two-dimensional electrophoresis.In sample preparation,overdrive centrifugation and sedimentation were used.During isoelectric focusing(IEF),different length of immobilized pH strips and different composition of rehydrating buffer were applied.And during sodium dodecyl sulfate-polyacrylamide gel electrophoresis(SDS-PAGE),the same 12%-concentration of PAGE was used.Silver nitrate was applied to stain proteins.Results showed that 2-dimensional maps were got.The feasibility of researching the immunity and other physiology phenomena of prawn were proved.Approaches of researching the immunity and other physiology phenomena of prawn were extremely extended.It′s helpful in revealing the pathogenesis of prawn by displaying some special proteins and offers some drug targets for the therapy of diseases.
【Fund】: 山东大学威海分校大学生科技项目(A06005)
【CateGory Index】: S917.4
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