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Experiments and Computer Simulation for Modulation of Stauntoniae on Sodium Channel in Rat Dorsal Root Ganglion Neurons

LI Xu,CHEN Su,LIU Xiangming(College of Electronic and Information Engineering,South-central University for Nationalities,Wuhan 430074,China)  
Using computer simulation to exclude other ion channels′s effect on patch clamp experiment results,the effect of injection stauntoniae on sodium channel currents and action potential in dorsal root ganglion neurons were observed to illuminate its analgesic mechanism.Hodgkin-Huxley model was used to fit the experimental data of whole cell patch clamp experiment which were recorded in dorsal root ganglion neurons in the absence and presence of 10%,25% and 50 % injection stauntoniae.Model parameters were estimated and fitting quality was calculated.Based on the parameters,neuron models were built.When parameters of the sodium channel were changed,the action potentials in dorsal root ganglion neurons in the absence and presence of 10%,25% and 50 % injection stauntoniae were generated by computer simulation.Patch clamp experimental results showed that injection stauntoniae inhibited the peak sodium currents in dorsal root ganglion neurons in a dose-dependent way and the activation and inactivation of the channels were influenced either.The results of computer simulation showed that the presence of injection stauntoniae shifted the half activation voltage of sodium channel to depolarization and the threshold activating action potential in dorsal root ganglion neuron increased.It proves that the analgesic effect of injection stauntoniae may be related to its interference with the transmission of nociceptive information in primary sensory neurons,which is caused by its inhibition on action potential in dorsal root ganglion neuron.
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