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The Assessment of Ectopic Osteoinduction by bBMP Using Micro-CT

LI Dan,LIU Min,HU Yunyu,BI Long,DUAN Yonghong,JIN Jing,WANG Jun,LIU Yin(Institute of Orthopaedics & Traumatology,Xijing Hospital,The Fourth Military Medical University,Xi'an 710032,China)  
To assess the 3-D image and changes in quality of osseous tissue produced through ectopic osteoinduction by bovine bone morphegenetic protein over an extended period by means of Micro-CT.Twenty-one Kunming rats(20±2)g were used, 2 mg bBMP were implanted into thigh muscles of both sides of the rat under anesthesia.Three animals of each were sacrificed at 1,2,4,6,8,10 and 12 weeks after the implantation.After being harvested,the newly formed bone tissue was fixed with 5% glutaraldehyde,then successively subjected to Micro-CT scan and 3-D reconstruction,measurement of tissue mineral consent(TMC) using ABA software and tissue mineral density(TMD),determination of bone volume fraction(BVF),structure model index(SMI),trabecular thickness(Tb.Th),trabecular number(Tb.N),cortical bone mineral density(BMD) and other parameters,3-D images were created and saved as TIFF files;statistical analyses were carried out by ANOVA(analysis of variance) using Statistics Package for Social Science(SPSS) software.At 2 weeks postimplantation,an oval mass of osseous tissue began to take shape;between 2 to 4 weeks,the ectopic-induced new bone presented as loose cancellous bone tissue,and the mineral content reached the first peak by the 4th week,showing numerous trabeculae;the innermost trabecular of the ectopic-induced lump of osseous tissue were resorbed with their number decreased with time(between 6th and 12th week),approaching the minimum by the 12th week;the outer part remodeled into cortical bone with TMC,Tb.Th,TMD and BMD of the lump as whole reaching the maximum by the 12th week.Bovine BMP possesses strong ectopic osteoinductivity,but the newly formed bone tissue is resorbed when the blood supply is inadequate,whereas the osseous tissue remodels with progression toward a mature tissue when provided with ample blood supply.
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